Efectos de la lesión química por estereotaxia del núcleo reticular del tálamo sobre la conducta de ratas en un laberinto en T después de la administración de 8-OH-DPAT

Pablo Andrade; Fiacro Jiménez; José Carrillo; Ylián Ramírez

doi:10.47924/neurotarget2008356

Authors

Pablo Andrade General Hospital of Mexico.
Fiacro Jiménez General Hospital of Mexico.
José Carrillo General Hospital of Mexico.
Ylián Ramírez General Hospital of Mexico.

DOI:

https://doi.org/10.47924/neurotarget2008356

Keywords:

T-maze, obsessive-compulsive disorder, psychosurgery, thalamus

Abstract

Introduction: 8-OH-DPAT is a selective 5HT presynaptic agonist useful in the perseverative T-maze model. Intramuscular administration of chlorimipramine (CMI) can prevent this effect. By other hand, the mechanism of action of neuromodulation in the orbitofrontal-thalamic system in psychiatric patients is not clear enough. Reticular thalamic nucleus (RTN) is part of the orbitofrontal-thalamic system then stereotactic lesion of it could be helpful to understand this new approach.

Material and methods: Thirty male Wistar rats were divided in 3 groups. Group 1 (Control) was only submitted to 8-OH-DPAT, group 2 (CMI) received the pharmacological treatment before 8-OH-DPAT administration and group 3 (RTN) were submitted to stereotactic bilateral lesion with AgNO3, one week before 5HT agonist administration. After specific treatment every group was evaluated into T-maze, measured by the number of perseverations in the same branch. Anatomic slices were obtained in order to verify the exact location of lesions. Statistical analysis was performed by t-Student paired for intragroup performance and ANOVA for intergroup measures.

Results: Significant differences (P < 0,05) intragroup were found between pre- and post injection of 8-OH-DPAT only in group 1. There was a significant difference (P < 0,05) intergroup in perseverations when compared the post 8-OH-DPAT state control versus the other groups.

Conclusion: RTN lesion had the same effect that CMI pretreatment group by preventing the 8-OH- DPAT action.

Metrics

Metrics Loading ...

References

American Psychiatric Association. Diagnostic and statistical manual of mental disorders: DSM-IV-TR. 4th ed. Washington DC: American Psychiatric Pub; 1994.

Millet B, Jaafari N. Traitement du trouble obsessionnel compulsif. [Treatment of obsessive-compulsive disorder][Article in French] Rev Prat. 2007;57(1):53-7.

Eddy KT, Dutra L, Bradley R, Westen D. A multidimensional meta-analysis of psychotherapy and pharmacotherapy for obsessive-compulsive disorder. Clin Psychol Rev. 2004;24(8):1011-30.

Fallon BA, Mathew SJ. Biological therapies for obsessive-compulsive disorder. J Psychiatr Pract. 2000;6(3):113-28.

Goddard AW, Shekhar A, Whiteman AF, McDougle CJ. Serotoninergic mechanisms in the treatment of obsessive-compulsive disorder. Drug Discov Today. 2008;13(7-8):325-32.

Flaisher-Grinberg S, Klavir O, Joel D. The role of 5-HT2A and 5-HT2C receptors in the signal attenuation rat model of obsessive-compulsive disorder. Int J Neuropsychopharmacol. 2008:1-15.

van Kuyck K, Demeulemeester H, Feys H, De Weerdt W, Dewil M, Tousseyn T, et al. Effects of electrical stimulation or lesion in nucleus accumbens on the behaviour of rats in a T-maze after administration of 8-OH-DPAT or vehicle. Behav Brain Res. 2003;140(1-2):165-73.

Ulloa RE, Nicolini H, Fernández-Guasti A. Age differences in an animal model of obsessive-compulsive disorder: participation of dopamine: dopamine in an animal model of OCD. Pharmacol Biochem Behav. 2004;78(4):661-6.

Yadin E, Friedman E, Bridger WH. Spontaneous alternation behavior: an animal model for obsessive-compulsive disorder? Pharmacol Biochem Behav. 1991;40(2):311-5.

Velasco M, Velasco F, Jiménez F, Carrillo-Ruiz JD, Velasco AL, Salín- Pascual R. Electrocortical and behavioral responses elicited by acute electrical stimulation of inferior thalamic peduncle and nucleus reticularis thalami in a patient with major depression disorder. Clin Neurophysiol. 2006;117(2):320-7.

Velasco F, Velasco M, Jiménez F, Velasco AL, Salín-Pascual R. Neurobiological background for performing surgical intervention in the inferior thalamic peduncle for treatment of major depression disorders. Neurosurgery. 2005;57(3):439-48.

Jiménez F, Velasco F, Salín-Pascual R, Velasco M, Nicolini H, Velasco AL, et al. Neuromodulation of the inferior thalamic peduncle for major depression and obsessive compulsive disorder. Acta Neurochir Suppl. 2007;97(Pt 2):393-8.

Velasco M, Lindsley DB. Role of orbital cortex in regulation of thalamocortical electrical activity. Science. 1965;149(690):1375-7.

Lipsman N, Neimat JS, Lozano AM. Deep brain stimulation for treatment refractory obsessive-compulsive disorder: the search for a valid target. Neurosurgery. 2007;61(1):1-11.

Paxinos G, Watson C. The Rat Brain in Stereotaxic Coordinates. San Diego: Academic Press; 1982.

Shiloh R, Nutt D, Weizman A. Atlas of Psychiatric Pharmacotherapy. 2nd ed. London, UK: Martin Dunitz; 2000.

Nuttin BJ, Gabriëls LA, Cosyns PR, Meyerson BA, Andréewitch S, Sunaert SG, et al. Long-term electrical capsular stimulation in patients with obsessive-compulsive disorder. Neurosurgery. 2003;52(6):1263-72.

Joel D, Doljansky J, Roz N, Rehavi M. Role of the orbital cortex and of the serotonergic system in a rat model of obsessive compulsive disorder. Neuroscience. 2005;130(1):25-36.